**United States Sales and Usage Limitation: For Research Use Only. Not for use in diagnostic procedures.

INTENDED USE

The HHV-8 IgG IFA assay is an in vitro immunological assay for the detection of anti-human herpesvirus 8 (HHV-8) specific IgG antibodies in human serum and plasma samples.

ASSAY PRINCIPLE

The Biotrin HHV-8 IFA system utilises the indirect immunofluorescent method of antibody detection and titre determination, providing both qualitative and quantitative results to the laboratory.

Patient samples are incubated with HHV-8 antigen, which has been stabilised on a glass slide. If HHV-8 IgG antibodies are present in the sample, a stable complex is formed between the antibody and the antigen on the slide.

Bound HHV-8 IgG antibody is then reacted with a fluorescein conjugated goat anti-human IgG and this complex is visualised with the aid of a fluorescence microscope.

Positive reactions are denoted by bright green fluorescence of infected cells. Uninfected cells are stained red due to the use of a counter stain

 Rapid & easy to use assay

 High levels of cells in each well

 Good representation of HHV-6 positive and negative cells in each well

 Positive and Negative controls included
 

ADDITIONAL INFORMATION

HHV-8:  Questions & Answers

HHV-8:  Patient Information

HHV-8:  Clinician Information

Instructions For Use

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